Gapdh #5174 cell signaling technology
WebCell Signaling Technology Inc gapdh cat 5174 antibodies. Gapdh Cat 5174 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars … WebFeb 18, 2024 · Proteins were detected using primary antibodies as follows: ACE2 (AF933, R&D Systems, Minneapolis, MN), TMPRSS2 (ab92323, Abcam, Cambridge, UK), furin (ab3467, Abcam), and GAPDH (5174, Cell Signaling Technology, Danvers, MA). Protein bands were quantified using ImageJ software (National Institutes of Health, Bethesda, MD).
Gapdh #5174 cell signaling technology
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WebWestern blot analysis of extracts from MC/9, NIH/3T3, and C2C12 cells using FcεRIA (E6D9L) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). … WebNov 15, 2024 · Immunoblotting was performed according to standard method with primary antibodies against TFEB (ab2636, Abcam), TFE3 (SAB4503154, Sigma), PD-L1 (17952-1-AP, ProteinTech), GAPDH (5174, Cell Signaling Technology), PCNA (AV03018, Sigma), BAX (2772, Cell Signaling Technology), phospho-S6 (4851, Cell Signaling …
WebOct 12, 2024 · The cell proliferation of BC cells was evaluated using the Cell Counting kit-8 (CCK-8). ZR-75-30 and BT474 cells in logarithmic growth phase were digested with 0.25% trypsin (Solarbio, T1300-100, Beijing, China) and then counted under a microscope (Shanghai Caikang Optical Instrument Co., XDS-500C) to prepare 3×10 4 cells/ml of cell … WebFeb 21, 2024 · Inflammation is an important risk factor in the development of inflammatory bowel disease (IBD) and colitis-associated colorectal cancer (CAC). Accumulating evidence indicates that some phytochemicals have anti-cancer properties. Polysaccharides extracted from Albuca bracteata (AB) have been reported to possess anti-neoplastic activities on …
WebGAPDH (D16H11) XP® Rabbit mAb. Catalog # Concentration Size List Price Quantity Your Price; 5174S 100 µl: $500.00 $450.00 Add to Cart: 5174T 20 µl: $209.00 $188.10 ... WebJun 21, 2024 · The primary antibodies used in this study were: GAPDH (5174, Cell Signaling Technology), β-Tubulin (YM3030, ImmunoWay), Vimentin (5741, Cell Signaling Technology), N-cad (4061, Cell Signaling Technology), Snail (3879, Cell Signaling Technology), Slug (9585, Cell Signaling Technology), E-cad (3195, Cell Signaling …
WebFeb 25, 2024 · Ferroptosis plays a key role in human cancer, but its function and mechanism in glioma is not clear. P62/SQSTM1 was reported to inhibit ferroptosis via the activation of NRF2 signaling pathway. In this study we reveal a dual role of p62 in ferroptosis of glioblastoma (GBM) according to p53 status. Lipid peroxidation analysis, …
WebThe official blog of Cell Signaling Technology (CST) where we discuss what to expect from your time at the bench, share tips, tricks, and information. ... Western blot analysis of extracts from various mouse tissues using GAPDH (D16H11) XP Rabbit mAB #5174. CST GAPDH antibodies for western blot with mouse reactivity: GAPDH (14C10) Rabbit mAb … marshall white md scWebDec 30, 2024 · Cell Culture and Treatment. The hep1-6 cell line was used to measure whether LPS treatment would change the expression level of FGFR1 and KLB. The cells were cultured in DMEM medium with 10% FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin at 37°C in a 5% CO 2 humidified incubator. The cells were plated in six-well … marshall white projects melbourneWebMar 16, 2024 · 13126 (Cell Signaling Technology) 1:1000: GAPDH: 5174 (Cell Signaling Technology) 1:2000: Open in a separate window. Invasion assay. An aliquot of 5 x 10 4 H1 cells was transferred to invasion chamber wells as previously described . After 24 h from initial plating, the medium was changed (2 ml in each chamber) to BAP treatment for … marshall white armadale vicWebApr 8, 2024 · HTB is associated with the decreased amount of CD8 + T cells in TME, (A-B) Box plots show the scores of immune signatures in the TCGA-COAD (A) and TCGA-SKCM (B) cohorts. Boxes represent 25%-75% of values, lines in boxes represent median values, whiskers represent 1.5 interquartile ranges, and black dots represent outliers, (C) IHC … marshall whitefieldWebGAPDH (D16H11) XP Rabbit mAb antibody . GAPDH human, mouse, rat, monkey (Cell Signaling Technology Cat# 5174, RRID:AB_10622025) monoclonal antibody. ... Cell Signaling Technology. 5174 also 5174P, 5174S, 5174T. 1; marshall white port melbourneWebFeb 11, 2024 · GAPDH (#5174) (1:5000) was selected as housekeeping protein for checking equal loading of each sample. After washing with 0.05% Tween-20 PBS, the membranes were incubated with the corresponding secondary antibodies (1:1000) (anti-rabbit, A0208; anti-mouse, A02616, Beyotime) for 2 h at room temperature. marshall white brighton properties for saleWebNov 28, 2016 · Cell Signaling Technology product type : antibody product name : GAPDH (D16H11) XP® Rabbit mAb catalog : 5174S quantity : 100 ul price : 260 USD clonality : … marshall wi fisheree