WebNov 21, 2024 · Under reduced conditions, the CBP-LA NPs showed redox-responsive behavior. The intracellular glutathione determination and the Pt-DNA adduct assay revealed that CBP-LA NPs could reduce the intracellular GSH levels and improve the efficiency of platinum chelating with DNA, which would overcome GSH-mediated … WebA clear yellow color was presented after mixing and keeping in room temperature for 5 minutes. The total thiol compound was quantified by reading the absorbance at 412 nm with a spectrophotometer, and comparing the absorbance of standard GSH (Sigma-Aldrich Co., St Louis, MO, USA). TBARs-MDA adduct assay
Formation, metabolism and transport of GSH-adducts …
Webmeasure the GSH conjugation of 1,2 epoxy-3(4-nitrophenoxy)-propane. The assay was also performed with rat liver cytosol. • Doubling the concentration of GSH used in the original protocol improved experimental repeatability for the micro-scale assay. CYP2E1 activity assay: Primary human urothelial cells and SV- WebNov 30, 2024 · One of the other challenges during heterologous expression of class I lanthipeptides in E. coli is the formation of glutathione (GSH) adducts. 30,34,35 Glutathione is a thiol-containing tripeptide that is involved in a variety of physiological functions in both mammals and bacteria. 40,41 Due to the relatively high concentration of GSH in E ... mylaps accessories
Characterization of a highly sensitive and selective
WebAt least, total reduced thiol concentrations (reflected by free NTB) are always higher than the GSH concentrations (reflected by GS-NTB adduct), when comparing only the results of the DTNB assay (compare Figures 4 … WebWe describe the development of an assay to detect glutathione adducts with combined high sensitivity, enhanced specificity, and rapid data analysis. In this assay, compounds were incubated with human liver … WebJan 22, 2024 · Yu and O'Connell improved the usual MS assay for GSH adducts (a neutral-loss scan for m/z 129) by using an offline nanoelectrospray ion source to improve MS … mylaps activeren